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HTPSELEX—a database of high-throughput SELEX libraries for transcription factor binding sites

机译:HTPSELEX-用于转录因子结合位点的高通量SELEX库的数据库

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摘要

HTPSELEX is a public database providing access to primary and derived data from high-throughput SELEX experiments aimed at characterizing the binding specificity of transcription factors. The resource is primarily intended to serve computational biologists interested in building models of transcription factor binding sites from large sets of binding sequences. The guiding principle is to make available all information that is relevant for this purpose. For each experiment, we try to provide accurate information about the protein material used, details of the wet lab protocol, an archive of sequencing trace files, assembled clone sequences (concatemers) and complete sets of in vitro selected protein-binding tags. In addition, we offer in-house derived binding sites models. HTPSELEX also offers reasonably large SELEX libraries obtained with conventional low-throughput protocols. The FTP site contains the trace archives and database flatfiles. The web server offers user-friendly interfaces for viewing individual entries and quality-controlled download of SELEX sequence libraries according to a user-defined sequencing quality threshold. HTPSELEX is available from and .
机译:HTPSELEX是一个公共数据库,可访问来自高通量SELEX实验的原始数据和派生数据,这些实验旨在表征转录因子的结合特异性。该资源主要用于为有兴趣从大量结合序列建立转录因子结合位点模型的计算生物学家提供服务。指导原则是提供与此目的相关的所有信息。对于每个实验,我们尝试提供有关所用蛋白质材料的准确信息,湿实验室方案的详细信息,测序跟踪文件的档案,组装的克隆序列(连接物)以及体外选择的蛋白质结合标签的完整集合。此外,我们提供内部衍生的结合位点模型。 HTPSELEX还提供通过常规低吞吐量协议获得的相当大的SELEX库。 FTP站点包含跟踪存档和数据库平面文件。 Web服务器提供了用户友好的界面,用于根据用户定义的测序质量阈值查看单个条目和SELEX序列库的质量控制下载。 HTPSELEX可从和获得。

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